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RNASeq Based Transcriptional Profiling of Pseudomonas aeruginosa PA14 after Short- and Long-Term Anoxic Cultivation in Synthetic Cystic Fibrosis Sputum Medium

Tata, Muralidhar; Wolfinger, Michael T.; Amman, Fabian; Roschanski, Nicole; Dötsch, Andreas; Sonnleitner, Elisabeth; Häussler, Susanne; Bläsi, Udo

Abstract:

The opportunistic human pathogen Pseudomonas aeruginosa can thrive under microaerophilic to anaerobic conditions in the lungs of cystic fibrosis patients. RNA$_{Seq}$ based comparative RNA profiling of the clinical isolate PA14 cultured in synthetic cystic fibrosis medium was performed after planktonic growth (OD$_{600}$ = 2.0; P), 30 min after shift to anaerobiosis (A-30) and after anaerobic biofilm growth for 96h (B-96) with the aim to reveal differentially regulated functions impacting on sustained anoxic biofilm formation as well as on tolerance towards different antibiotics. Most notably, functions involved in sulfur metabolism were found to be up-regulated in B-96 cells when compared to A-30 cells. Based on the transcriptome studies a set of transposon mutants were screened, which revealed novel functions involved in anoxic biofilm growth.In addition, these studies revealed a decreased and an increased abundance of the oprD and the mexCD-oprJ operon transcripts, respectively, in B-96 cells, which may explain their increased tolerance towards meropenem and to antibiotics that are expelled by the MexCD-OprD efflux pump. The OprI protein has been implicated as a target for cationic antimicrobial peptides, such as SMAP-29. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000064596
Originalveröffentlichung
DOI: 10.1371/journal.pone.0147811
Scopus
Zitationen: 31
Web of Science
Zitationen: 29
Dimensions
Zitationen: 39
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2016
Sprache Englisch
Identifikator ISSN: 1932-6203
urn:nbn:de:swb:90-645965
KITopen-ID: 1000064596
HGF-Programm 47.02.06 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IFG
Erschienen in PLoS one
Verlag Public Library of Science (PLoS)
Band 11
Heft 1
Seiten e0147811
Vorab online veröffentlicht am 28.01.2016
Nachgewiesen in Scopus
PubMed
Dimensions
Web of Science
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