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Melanosomes in pigmented epithelia maintain eye lens transparency during zebrafish embryonic development

Takamiya, M. 1; Xu, F. 2; Suhonen, H.; Gourain, V. 1; Yang, L. 1; Yu Ho, N.; Helfen, L. 2; Schröck, A. 1; Etard, C. 1; Grabher, C. 1; Rastegar, S. ORCID iD icon 1; Schlunck, G.; Reinhard, T.; Baumbach, T. 2; Strähle, U. 1
1 Institut für Toxikologie und Genetik (ITG), Karlsruher Institut für Technologie (KIT)
2 Institut für Photonenforschung und Synchrotronstrahlung (IPS), Karlsruher Institut für Technologie (KIT)

Abstract (englisch):

Altered levels of trace elements are associated with increased oxidative stress that is eventually responsible for pathologic conditions. Oxidative stress has been proposed to be involved in eye diseases, including cataract formation. We visualized the distribution of metals and other trace elements in the eye of zebrafish embryos by micro X-ray fluorescence (μ-XRF) imaging. Many elements showed highest accumulation in the retinal pigment epithelium (RPE) of the zebrafish embryo. Knockdown of the zebrafish brown locus homologues tyrp1a/b eliminated accumulation of these elements in the RPE, indicating that they are bound by mature melanosomes. Furthermore, albino (slc45a2) mutants, which completely lack melanosomes, developed abnormal lens reflections similar to the congenital cataract caused by mutation of the myosin chaperon Unc45b, and an in situ spin trapping assay revealed increased oxidative stress in the lens of albino mutants. Finally transplanting a wildtype lens into an albino mutant background resulted in cataract formation. These data suggest that melanosomes in pigment epithelial cells protect the lens from oxidative stress during embryonic development, likely by buffering trace elements.


Volltext §
DOI: 10.5445/IR/1000054667
Originalveröffentlichung
DOI: 10.1038/srep25046
Scopus
Zitationen: 9
Web of Science
Zitationen: 9
Dimensions
Zitationen: 9
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Photonenforschung und Synchrotronstrahlung (IPS)
Institut für Toxikologie und Genetik (ITG)
Laboratorium für Applikationen der Synchrotronstrahlung (LAS)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2016
Sprache Englisch
Identifikator ISSN: 2045-2322
urn:nbn:de:swb:90-546670
KITopen-ID: 1000054667
HGF-Programm 47.01.01 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Erschienen in Scientific reports
Verlag Nature Research
Band 6
Seiten Art. Nr.: 25046
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 04.05.2016
Schlagwörter Embryonic induction; Mechanisms of disease; X-ray tomography
Nachgewiesen in Web of Science
Scopus
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