Cell aggregation of Pseudomonas aeruginosa strain PAO1 as an energy-dependent stress response during growth sodium dodecyl sulfate

Lade...
Vorschaubild
Dateien
Cell_aggregation_of_Pseudomonas_aeruginosa_2006.pdf
Cell_aggregation_of_Pseudomonas_aeruginosa_2006.pdfGröße: 433.52 KBDownloads: 742
Datum
2006
Autor:innen
Klebensberger, Janosch
Rui, Oliver
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Archives of Microbiology. 2006, 185(6), pp. 417-427. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s00203-006-0111-y
Zusammenfassung

Pseudomonas aeruginosa strain PAO1 grew with the detergent sodium dodecyl sulfate (SDS). The growth started with the formation of macroscopic cell aggregates which consisted of respiring cells embedded in an extracellular matrix composed of acidic polysaccharides and DNA. Damaged and uncultivable cells accumulated in these aggregates compared to those cells that remained suspended. We investigated the response of suspended cells to SDS under different conditions. At high energy supply, the cells responded with a decrease in optical density and in viable counts, release of protein and DNA, and formation of macroscopic aggregates. This response was not observed if the energy supply was reduced by inhibiting respiration with KCN, or if cells not induced for SDS degradation were exposed to SDS. Exposure to SDS caused cell lysis without aggregation if cells were completely deprived of energy, either by applying anoxic conditions, by addition of CCCP, or by addition of KCN to a mutant defective in cyanideinsensitive respiration. Aggregated cells showed a more than 100-fold higher survival rate after exposure to SDS plus CCCP than suspended cells. Our results demonstrate that cell aggregation is an energy-dependent response of P. aeruginosa to detergent stress which might serve as a survival strategy during growth with SDS.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Cell aggregation, Pseudomonas aeruginosa, SDS, Detergent degradation, Stress response
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690KLEBENSBERGER, Janosch, Oliver RUI, Eva FRITZ, Bernhard SCHINK, Bodo PHILIPP, 2006. Cell aggregation of Pseudomonas aeruginosa strain PAO1 as an energy-dependent stress response during growth sodium dodecyl sulfate. In: Archives of Microbiology. 2006, 185(6), pp. 417-427. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s00203-006-0111-y
BibTex
@article{Klebensberger2006aggre-8536,
  year={2006},
  doi={10.1007/s00203-006-0111-y},
  title={Cell aggregation of Pseudomonas aeruginosa strain PAO1 as an energy-dependent stress response during growth sodium dodecyl sulfate},
  number={6},
  volume={185},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={417--427},
  author={Klebensberger, Janosch and Rui, Oliver and Fritz, Eva and Schink, Bernhard and Philipp, Bodo}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8536">
    <dc:creator>Rui, Oliver</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Klebensberger, Janosch</dc:creator>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8536/1/Cell_aggregation_of_Pseudomonas_aeruginosa_2006.pdf"/>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8536"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 185 (2006), 6, pp. 417-427</dcterms:bibliographicCitation>
    <dc:language>eng</dc:language>
    <dcterms:abstract xml:lang="eng">Pseudomonas aeruginosa strain PAO1 grew with the detergent sodium dodecyl sulfate (SDS). The growth started with the formation of macroscopic cell aggregates which consisted of respiring cells embedded in an extracellular matrix composed of acidic polysaccharides and DNA. Damaged and uncultivable cells accumulated in these aggregates compared to those cells that remained suspended. We investigated the response of suspended cells to SDS under different conditions. At high energy supply, the cells responded with a decrease in optical density and in viable counts, release of protein and DNA, and formation of macroscopic aggregates. This response was not observed if the energy supply was reduced by inhibiting respiration with KCN, or if cells not induced for SDS degradation were exposed to SDS. Exposure to SDS caused cell lysis without aggregation if cells were completely deprived of energy, either by applying anoxic conditions, by addition of CCCP, or by addition of KCN to a mutant defective in cyanideinsensitive respiration. Aggregated cells showed a more than 100-fold higher survival rate after exposure to SDS plus CCCP than suspended cells. Our results demonstrate that cell aggregation is an energy-dependent response of P. aeruginosa to detergent stress which might serve as a survival strategy during growth with SDS.</dcterms:abstract>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Schink, Bernhard</dc:creator>
    <dc:creator>Fritz, Eva</dc:creator>
    <dcterms:issued>2006</dcterms:issued>
    <dc:contributor>Schink, Bernhard</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:44:28Z</dc:date>
    <dc:contributor>Rui, Oliver</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8536/1/Cell_aggregation_of_Pseudomonas_aeruginosa_2006.pdf"/>
    <dc:creator>Philipp, Bodo</dc:creator>
    <dc:contributor>Fritz, Eva</dc:contributor>
    <dc:format>application/pdf</dc:format>
    <dc:contributor>Klebensberger, Janosch</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:44:28Z</dcterms:available>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Philipp, Bodo</dc:contributor>
    <dcterms:title>Cell aggregation of Pseudomonas aeruginosa strain PAO1 as an energy-dependent stress response during growth sodium dodecyl sulfate</dcterms:title>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen