Yeast vacuoles fragment in an asymmetrical two-phase process with distinct protein requirements.

Details

Ressource 1Download: BIB_FB907E92D5FC.P001.pdf (5808.58 [Ko])
State: Public
Version: author
Serval ID
serval:BIB_FB907E92D5FC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Yeast vacuoles fragment in an asymmetrical two-phase process with distinct protein requirements.
Journal
Molecular Biology of the Cell
Author(s)
Zieger M., Mayer A.
ISSN
1939-4586 (Electronic)
ISSN-L
1059-1524
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
23
Number
17
Pages
3438-3449
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Abstract
Yeast vacuoles fragment and fuse in response to environmental conditions, such as changes in osmotic conditions or nutrient availability. Here we analyze osmotically induced vacuole fragmentation by time-lapse microscopy. Small fragmentation products originate directly from the large central vacuole. This happens by asymmetrical scission rather than by consecutive equal divisions. Fragmentation occurs in two distinct phases. Initially, vacuoles shrink and generate deep invaginations that leave behind tubular structures in their vicinity. Already this invagination requires the dynamin-like GTPase Vps1p and the vacuolar proton gradient. Invaginations are stabilized by phosphatidylinositol 3-phosphate (PI(3)P) produced by the phosphoinositide 3-kinase complex II. Subsequently, vesicles pinch off from the tips of the tubular structures in a polarized manner, directly generating fragmentation products of the final size. This phase depends on the production of phosphatidylinositol-3,5-bisphosphate and the Fab1 complex. It is accelerated by the PI(3)P- and phosphatidylinositol 3,5-bisphosphate-binding protein Atg18p. Thus vacuoles fragment in two steps with distinct protein and lipid requirements.
Pubmed
Web of science
Open Access
Yes
Create date
10/01/2013 18:12
Last modification date
20/08/2019 16:26
Usage data