Odorant binding proteins of the red imported fire ant, Solenopsis invicta: an example of the problems facing the analysis of widely divergent proteins.

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Version: Final published version
Serval ID
serval:BIB_EE4581E2AF58
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Odorant binding proteins of the red imported fire ant, Solenopsis invicta: an example of the problems facing the analysis of widely divergent proteins.
Journal
PLoS One
Author(s)
Gotzek D., Robertson H.M., Wurm Y., Shoemaker D.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
6
Number
1
Pages
e16289
Language
english
Abstract
We describe the odorant binding proteins (OBPs) of the red imported fire ant, Solenopsis invicta, obtained from analyses of an EST library and separate 454 sequencing runs of two normalized cDNA libraries. We identified a total of 18 putative functional OBPs in this ant. A third of the fire ant OBPs are orthologs to honey bee OBPs. Another third of the OBPs belong to a lineage-specific expansion, which is a common feature of insect OBP evolution. Like other OBPs, the different fire ant OBPs share little sequence similarity (∼ 20%), rendering evolutionary analyses difficult. We discuss the resulting problems with sequence alignment, phylogenetic analysis, and tests of selection. As previously suggested, our results underscore the importance for careful exploration of the sensitivity to the effects of alignment methods for data comprising widely divergent sequences.
Keywords
Amino Acid Sequence, Animals, Ants/chemistry, Evolution, Molecular, Gene Library, Phylogeny, Receptors, Odorant/analysis, Receptors, Odorant/genetics, Sequence Alignment
Pubmed
Web of science
Open Access
Yes
Create date
02/02/2011 9:26
Last modification date
20/08/2019 16:15
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