Ultrastructural evidence of exosome secretion by progenitor cells in adult mouse myocardium and adult human cardiospheres.

Details

Ressource 1Download: BIB_E6BB61D40B8C.P001.pdf (3220.75 [Ko])
State: Public
Version: author
Serval ID
serval:BIB_E6BB61D40B8C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ultrastructural evidence of exosome secretion by progenitor cells in adult mouse myocardium and adult human cardiospheres.
Journal
Journal of Biomedicine and Biotechnology
Author(s)
Barile L., Gherghiceanu M., Popescu L.M., Moccetti T., Vassalli G.
ISSN
1110-7251 (Electronic)
ISSN-L
1110-7243
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
2012
Pages
354605
Language
english
Notes
Publication types: Journal Article WOS Document Type: Review
Abstract
The demonstration of beneficial effects of cell therapy despite the persistence of only few transplanted cells in vivo suggests secreted factors may be the active component of this treatment. This so-called paracrine hypothesis is supported by observations that culture media conditioned by progenitor cells contain growth factors that mediate proangiogenic and cytoprotective effects. Cardiac progenitor cells in semi-suspension culture form spherical clusters (cardiospheres) that deliver paracrine signals to neighboring cells. A key component of paracrine secretion is exosomes, membrane vesicles that are stored intracellularly in endosomal compartments and are secreted when these structures fuse with the cell plasma membrane. Exosomes have been identified as the active component of proangiogenic effects of bone marrow CD34(+) stem cells in mice and the regenerative effects of embryonic mesenchymal stem cells in infarcted hearts in pigs and mice. Here, we provide electron microscopic evidence of exosome secretion by progenitor cells in mouse myocardium and human cardiospheres. Exosomes are emerging as an attractive vector of paracrine signals delivered by progenitor cells. They can be stored as an "off-the-shelf" product. As such, exosomes have the potential for circumventing many of the limitations of viable cells for therapeutic applications in regenerative medicine.
Pubmed
Web of science
Open Access
Yes
Create date
20/12/2012 18:57
Last modification date
20/08/2019 16:09
Usage data