Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission.

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Serval ID
serval:BIB_D85163C5EE09
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission.
Journal
Journal of Neuroscience
Author(s)
Martineau M., Shi T., Puyal J., Knolhoff A.M., Dulong J., Gasnier B., Klingauf J., Sweedler J.V., Jahn R., Mothet J.P.
ISSN
1529-2401 (Electronic)
ISSN-L
0270-6474
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
33
Number
8
Pages
3413-3423
Language
english
Abstract
Glial cells are increasingly recognized as active players that profoundly influence neuronal synaptic transmission by specialized signaling pathways. In particular, astrocytes have been shown recently to release small molecules, such as the amino acids l-glutamate and d-serine as "gliotransmitters," which directly control the efficacy of adjacent synapses. However, it is still controversial whether gliotransmitters are released from a cytosolic pool or by Ca(2+)-dependent exocytosis from secretory vesicles, i.e., by a mechanism similar to the release of synaptic vesicles in synapses. Here we report that rat cortical astrocytes contain storage vesicles that display morphological and biochemical features similar to neuronal synaptic vesicles. These vesicles share some, but not all, membrane proteins with synaptic vesicles, including the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) synaptobrevin 2, and contain both l-glutamate and d-serine. Furthermore, they show uptake of l-glutamate and d-serine that is driven by a proton electrochemical gradient. d-Serine uptake is associated with vesicle acidification and is dependent on chloride. Whereas l-serine is not transported, serine racemase, the synthesizing enzyme for d-serine, is anchored to the membrane of the vesicles, allowing local generation of d-serine. Finally, we reveal a previously unexpected mutual vesicular synergy between d-serine and l-glutamate filling in glia vesicles. We conclude that astrocytes contain vesicles capable of storing and releasing d-serine, l-glutamate, and most likely other neuromodulators in an activity-dependent manner.
Keywords
Animals, Astrocytes/metabolism, Astrocytes/ultrastructure, Biological Transport, Active/physiology, Cells, Cultured, Female, Male, Neuroglia/metabolism, Neuroglia/physiology, Rats, Rats, Sprague-Dawley, Rats, Wistar, Serine/metabolism, Synaptic Transmission/physiology, Synaptic Vesicles/metabolism, Synaptic Vesicles/ultrastructure
Pubmed
Web of science
Open Access
Yes
Create date
01/07/2013 10:07
Last modification date
20/08/2019 15:57
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