Discovery of a 29-gene panel in peripheral blood mononuclear cells for the detection of colorectal cancer and adenomas using high throughput real-time PCR.

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Serval ID
serval:BIB_D568005E782F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Discovery of a 29-gene panel in peripheral blood mononuclear cells for the detection of colorectal cancer and adenomas using high throughput real-time PCR.
Journal
Plos One
Author(s)
Ciarloni L., Hosseinian S., Monnier-Benoit S., Imaizumi N., Dorta G., Ruegg C.
Working group(s)
DGNP-COL-0310 Study Group
Contributor(s)
Song SY., Kim JS., Myung SJ., Borovicka J., Meier R., van der Weg B., Beglinger C.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
10
Number
4
Pages
e0123904
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't Publication Status: epublish
Abstract
Colorectal cancer (CRC) is the second leading cause of cancer-related death in developed countries. Early detection of CRC leads to decreased CRC mortality. A blood-based CRC screening test is highly desirable due to limited invasiveness and high acceptance rate among patients compared to currently used fecal occult blood testing and colonoscopy. Here we describe the discovery and validation of a 29-gene panel in peripheral blood mononuclear cells (PBMC) for the detection of CRC and adenomatous polyps (AP). Blood samples were prospectively collected from a multicenter, case-control clinical study. First, we profiled 93 samples with 667 candidate and 3 reference genes by high throughput real-time PCR (OpenArray system). After analysis, 160 genes were retained and tested again on 51 additional samples. Low expressed and unstable genes were discarded resulting in a final dataset of 144 samples profiled with 140 genes. To define which genes, alone or in combinations had the highest potential to discriminate AP and/or CRC from controls, data were analyzed by a combination of univariate and multivariate methods. A list of 29 potentially discriminant genes was compiled and evaluated for its predictive accuracy by penalized logistic regression and bootstrap. This method discriminated AP >1cm and CRC from controls with a sensitivity of 59% and 75%, respectively, with 91% specificity. The behavior of the 29-gene panel was validated with a LightCycler 480 real-time PCR platform, commonly adopted by clinical laboratories. In this work we identified a 29-gene panel expressed in PBMC that can be used for developing a novel minimally-invasive test for accurate detection of AP and CRC using a standard real-time PCR platform.
Keywords
Adenoma/blood, Adenoma/diagnosis, Adenomatous Polyps/blood, Adenomatous Polyps/diagnosis, Aged, Biomarkers, Tumor/blood, Biomarkers, Tumor/genetics, Case-Control Studies, Colonoscopy, Colorectal Neoplasms/blood, Colorectal Neoplasms/diagnosis, Early Detection of Cancer, Female, Humans, Leukocytes, Mononuclear/metabolism, Middle Aged, Prospective Studies, Real-Time Polymerase Chain Reaction
Pubmed
Web of science
Open Access
Yes
Create date
19/05/2015 17:22
Last modification date
20/08/2019 15:55
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