Precision and scaling in morphogen gradient read-out.

Details

Ressource 1Download: BIB_C6BCC0E0674B.P001.pdf (439.60 [Ko])
State: Public
Version: author
Serval ID
serval:BIB_C6BCC0E0674B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Precision and scaling in morphogen gradient read-out.
Journal
Molecular Systems Biology
Author(s)
de Lachapelle Aitana Morton, Bergmann Sven
ISSN
1744-4292[electronic], 1744-4292[linking]
Publication state
Published
Issued date
2010
Volume
6
Pages
351
Language
english
Abstract
Morphogen gradients infer cell fate as a function of cellular position. Experiments in Drosophila embryos have shown that the Bicoid (Bcd) gradient is precise and exhibits some degree of scaling. We present experimental results on the precision of Bcd target genes for embryos with a single, double or quadruple dose of bicoid demonstrating that precision is highest at mid-embryo and position dependent, rather than gene dependent. This confirms that the major contribution to precision is achieved already at the Bcd gradient formation. Modeling this dynamic process, we investigate precision for inter-embryo fluctuations in different parameters affecting gradient formation. Within our modeling framework, the observed precision can only be achieved by a transient Bcd profile. Studying different extensions of our modeling framework reveals that scaling is generally position dependent and decreases toward the posterior pole. Our measurements confirm this trend, indicating almost perfect scaling except for anterior most expression domains, which overcompensate fluctuations in embryo length.
Keywords
Bicoid, Drosophila, Morphogen Gradients, Precision, Scaling, Early Drosophila Embryo, Positional Information, Bicoid Gradient, Establishment, Protein, Melanogaster, Proportions, Diffusion, Dynamics, Pattern
Pubmed
Web of science
Open Access
Yes
Create date
23/04/2010 10:06
Last modification date
20/08/2019 16:42
Usage data