Distinct levels in Pom1 gradients limit Cdr2 activity and localization to time and position division.

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Version: Final published version
Serval ID
serval:BIB_BA90E254E2C0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Distinct levels in Pom1 gradients limit Cdr2 activity and localization to time and position division.
Journal
Cell Cycle
Author(s)
Bhatia P., Hachet O., Hersch M., Rincon S.A., Berthelot-Grosjean M., Dalessi S., Basterra L., Bergmann S., Paoletti A., Martin S.G.
ISSN
1551-4005 (Electronic)
ISSN-L
1551-4005
Publication state
Published
Issued date
2014
Volume
13
Number
4
Pages
538-552
Language
english
Abstract
Where and when cells divide are fundamental questions. In rod-shaped fission yeast cells, the DYRK-family kinase Pom1 is organized in concentration gradients from cell poles and controls cell division timing and positioning. Pom1 gradients restrict to mid-cell the SAD-like kinase Cdr2, which recruits Mid1/Anillin for medial division. Pom1 also delays mitotic commitment through Cdr2, which inhibits Wee1. Here, we describe quantitatively the distributions of cortical Pom1 and Cdr2. These reveal low profile overlap contrasting with previous whole-cell measurements and Cdr2 levels increase with cell elongation, raising the possibility that Pom1 regulates mitotic commitment by controlling Cdr2 medial levels. However, we show that distinct thresholds of Pom1 activity define the timing and positioning of division. Three conditions-a separation-of-function Pom1 allele, partial downregulation of Pom1 activity, and haploinsufficiency in diploid cells-yield cells that divide early, similar to pom1 deletion, but medially, like wild-type cells. In these cells, Cdr2 is localized correctly at mid-cell. Further, Cdr2 overexpression promotes precocious mitosis only in absence of Pom1. Thus, Pom1 inhibits Cdr2 for mitotic commitment independently of regulating its localization or cortical levels. Indeed, we show Pom1 restricts Cdr2 activity through phosphorylation of a C-terminal self-inhibitory tail. In summary, our results demonstrate that distinct levels in Pom1 gradients delineate a medial Cdr2 domain, for cell division placement, and control its activity, for mitotic commitment.
Keywords
fission, cell growth, Pom1 DYRK kinase, yeast, cell division, gradient, cell cycle, Schizosaccharomyces pombe
Pubmed
Web of science
Create date
12/12/2013 22:02
Last modification date
20/08/2019 15:28
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