Glucocorticoid regulation of mouse mammary tumor virus: identification of a short essential DNA region

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Type
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Publications
Institution
Title
Glucocorticoid regulation of mouse mammary tumor virus: identification of a short essential DNA region
Journal
EMBO Journal
Author(s)
Buetti E., Diggelmann H.
ISSN
0261-4189 (Print)
Publication state
Published
Issued date
1983
Volume
2
Number
8
Pages
1423-1429
Notes
Journal Article Research Support, Non-U.S. Gov't
Abstract
Transcription of mouse mammary tumor virus (MMTV) DNA is stimulated by steroid hormones. To determine the DNA sequences involved in this regulation, we constructed a plasmid containing the MMTV long terminal repeat (LTR) in front of the coding region of the herpes simplex thymidine kinase gene, from which the promoter had been removed. Portions of the LTR were removed by the nuclease Ba/31, and the deleted molecules were recloned and tested for transcriptional activity in transfections of Ltk-aprt- cells. Stably transfected cell clones were selected and hormone-dependent transcription from the MMTV promoter was studied by the S1 nuclease mapping method. The results show that DNA sequences between -105 and -204 base pairs upstream from the initiation site of viral transcription are required for glucocorticoid stimulation.
Keywords
Animals Base Sequence *DNA, Viral Gene Expression Regulation, Viral/*drug effects Glucocorticoids/*pharmacology L Cells (Cell Line) Mammary Tumor Virus, Mouse/*genetics Mice Molecular Sequence Data Plasmids Recombination, Genetic Simplexvirus/enzymology/genetics Thymidine Kinase/genetics Transcription, Genetic Transfection
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Web of science
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25/01/2008 14:25
Last modification date
20/08/2019 15:15
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