Manipulating the sensitivity of signal-induced repression: quantification and consequences of altered brinker gradients.

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Serval ID
serval:BIB_A84BFC496D76
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Manipulating the sensitivity of signal-induced repression: quantification and consequences of altered brinker gradients.
Journal
Plos One
Author(s)
Gafner L., Dalessi S., Escher E., Pyrowolakis G., Bergmann S., Basler K.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2013
Volume
8
Number
8
Pages
e71224
Language
english
Notes
Publication types: Journal Article
Abstract
Traditionally, the analysis of gene regulatory regions suffered from the caveat that it was restricted to artificial contexts (e.g. reporter constructs of limited size). With the advent of the BAC recombineering technique, genomic constructs can now be generated to test regulatory elements in their endogenous environment. The expression of the transcriptional repressor brinker (brk) is negatively regulated by Dpp signaling. Repression is mediated by small sequence motifs, the silencer elements (SEs), that are present in multiple copies in the regulatory region of brk. In this work, we manipulated the SEs in the brk locus. We precisely quantified the effects of the individual SEs on the Brk gradient in the wing disc by employing a 1D data extraction method, followed by the quantification of the data with reference to an internal control. We found that mutating the SEs results in an expansion of the brk expression domain. However, even after mutating all predicted SEs, repression could still be observed in regions of maximal Dpp levels. Thus, our data point to the presence of additional, low affinity binding sites in the brk locus.
Pubmed
Web of science
Open Access
Yes
Create date
20/09/2013 17:24
Last modification date
20/08/2019 15:12
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