Models that include supercoiling of topological domains reproduce several known features of interphase chromosomes.

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Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Models that include supercoiling of topological domains reproduce several known features of interphase chromosomes.
Journal
Nucleic Acids Research
Author(s)
Benedetti F., Dorier J., Burnier Y., Stasiak A.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Publication state
Published
Issued date
2014
Volume
42
Number
5
Pages
2848-2855
Language
english
Abstract
Understanding the structure of interphase chromosomes is essential to elucidate regulatory mechanisms of gene expression. During recent years, high-throughput DNA sequencing expanded the power of chromosome conformation capture (3C) methods that provide information about reciprocal spatial proximity of chromosomal loci. Since 2012, it is known that entire chromatin in interphase chromosomes is organized into regions with strongly increased frequency of internal contacts. These regions, with the average size of ∼1 Mb, were named topological domains. More recent studies demonstrated presence of unconstrained supercoiling in interphase chromosomes. Using Brownian dynamics simulations, we show here that by including supercoiling into models of topological domains one can reproduce and thus provide possible explanations of several experimentally observed characteristics of interphase chromosomes, such as their complex contact maps.
Pubmed
Web of science
Open Access
Yes
Create date
06/02/2014 15:02
Last modification date
20/08/2019 15:11
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