Nitric oxide sustains IL-1β expression in human dendritic cells enhancing their capacity to induce IL-17-producing T-cells.

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Serval ID
serval:BIB_A131D9ECEED0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Nitric oxide sustains IL-1β expression in human dendritic cells enhancing their capacity to induce IL-17-producing T-cells.
Journal
Plos One
Author(s)
Obregon C., Graf L., Chung K.F., Cesson V., Nicod L.P.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
10
Number
4
Pages
e0120134
Language
english
Abstract
The role played by lung dendritic cells (DCs) which are influenced by external antigens and by their redox state in controlling inflammation is unclear. We studied the role played by nitric oxide (NO) in DC maturation and function. Human DCs were stimulated with a long-acting NO donor, DPTA NONOate, prior to exposure to lipopolysaccharide (LPS). Dose-and time-dependent experiments were performed with DCs with the aim of measuring the release and gene expression of inflammatory cytokines capable of modifying T-cell differentiation, towardsTh1, Th2 and Th17 cells. NO changed the pattern of cytokine release by LPS-matured DCs, dependent on the concentration of NO, as well as on the timing of its addition to the cells during maturation. Addition of NO before LPS-induced maturation strongly inhibited the release of IL-12, while increasing the expression and release of IL-23, IL-1β and IL-6, which are all involved in Th17 polarization. Indeed, DCs treated with NO efficiently induced the release of IL-17 by T-cells through IL-1β. Our work highlights the important role that NO may play in sustaining inflammation during an infection through the preferential differentiation of the Th17 lineage.
Keywords
Alkenes/pharmacology, Benzoates/pharmacology, Dendritic Cells/drug effects, Dendritic Cells/immunology, Dose-Response Relationship, Drug, Gene Expression Regulation/drug effects, Humans, Imidazoles/pharmacology, Interleukin-17/biosynthesis, Interleukin-17/secretion, Interleukin-1beta/genetics, Nitric Oxide/pharmacology, T-Lymphocytes/drug effects, T-Lymphocytes/immunology, Time Factors
Pubmed
Web of science
Open Access
Yes
Create date
12/02/2016 14:01
Last modification date
20/08/2019 15:07
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