Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based trafficking.

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State: Public
Version: Final published version
Serval ID
serval:BIB_9B4198FCF94C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based trafficking.
Journal
PLoS Biology
Author(s)
Bendezú F.O., Vincenzetti V., Vavylonis D., Wyss R., Vogel H., Martin S.G.
ISSN
1545-7885 (Electronic)
ISSN-L
1544-9173
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
13
Number
4
Pages
e1002097
Language
english
Abstract
The small Rho-family GTPase Cdc42 is critical for cell polarization and polarizes spontaneously in absence of upstream spatial cues. Spontaneous polarization is thought to require dynamic Cdc42 recycling through Guanine nucleotide Dissociation Inhibitor (GDI)-mediated membrane extraction and vesicle trafficking. Here, we describe a functional fluorescent Cdc42 allele in fission yeast, which demonstrates Cdc42 dynamics and polarization independent of these pathways. Furthermore, an engineered Cdc42 allele targeted to the membrane independently of these recycling pathways by an amphipathic helix is viable and polarizes spontaneously to multiple sites in fission and budding yeasts. We show that Cdc42 is highly mobile at the membrane and accumulates at sites of activity, where it displays slower mobility. By contrast, a near-immobile transmembrane domain-containing Cdc42 allele supports viability and polarized activity, but does not accumulate at sites of activity. We propose that Cdc42 activation, enhanced by positive feedback, leads to its local accumulation by capture of fast-diffusing inactive molecules.
Keywords
Actins/metabolism, Alleles, Cell Polarity, Fluorescent Dyes, Guanine Nucleotide Dissociation Inhibitors/metabolism, Protein Transport, Schizosaccharomyces/cytology, Schizosaccharomyces/metabolism, cdc42 GTP-Binding Protein/genetics, cdc42 GTP-Binding Protein/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
12/06/2015 16:48
Last modification date
20/08/2019 15:02
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