Production of Pseudomonas aeruginosa Intercellular Small Signaling Molecules in Human Burn Wounds

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Serval ID
serval:BIB_8DC43020C77D
Type
Article: article from journal or magazin.
Publication sub-type
Case report (case report): feedback on an observation with a short commentary.
Collection
Publications
Institution
Title
Production of Pseudomonas aeruginosa Intercellular Small Signaling Molecules in Human Burn Wounds
Journal
Journal of Pathogens
Author(s)
Que Y.A., Hazan R., Ryan C.M., Milot S., Lépine F., Lydon M., Rahme L.G.
ISSN
2090-3065
ISSN-L
2090-3065
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
2011
Number
ID 549302
Pages
1-5
Language
english
Abstract
Pseudomonas aeruginosa has developed a complex cell-to-cell communication system that relies on low-molecular weight excreted molecules to control the production of its virulence factors. We previously characterized the transcriptional regulator MvfR, that controls a major network of acute virulence functions in P. aeruginosa through the control of its ligands, the 4-hydroxy-2-alkylquinolines (HAQs)-4-hydroxy-2-heptylquinoline (HHQ) and 3,4-dihydroxy-2-heptylquinoline (PQS). Though HHQ and PQS are produced in infected animals, their ratios differ from those in bacterial cultures. Because these molecules are critical for the potency of activation of acute virulence functions, here we investigated whether they are also produced during human P. aeruginosa acute wound infection and whether their ratio is similar to that observed in P. aeruginosa-infected mice. We found that a clinically relevant P. aeruginosa isolate produced detectable levels of HAQs with ratios of HHQ and PQS that were similar to those produced in burned and infected animals, and not resembling ratios in bacterial cultures. These molecules could be isolated from wound tissue as well as from drainage liquid. These results demonstrate for the first time that HAQs can be isolated and quantified from acute human wound infection sites and validate the relevance of previous studies conducted in mammalian models of infection.
Open Access
Yes
Create date
02/11/2011 17:16
Last modification date
20/08/2019 14:51
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