The pancreas responds to remote damage and systemic stress by secretion of the pancreatic secretory proteins PSP/regI and PAP/regIII.

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State: Public
Version: Final published version
Serval ID
serval:BIB_6D9A87A5E49E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The pancreas responds to remote damage and systemic stress by secretion of the pancreatic secretory proteins PSP/regI and PAP/regIII.
Journal
Oncotarget
Author(s)
Reding T., Palmiere C., Pazhepurackel C., Schiesser M., Bimmler D., Schlegel A., Süss U., Steiner S., Mancina L., Seleznik G., Graf R.
ISSN
1949-2553 (Electronic)
ISSN-L
1949-2553
Publication state
Published
Issued date
02/05/2017
Peer-reviewed
Oui
Volume
8
Number
18
Pages
30162-30174
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
In patients with infection and sepsis serum levels of Pancreatic Stone protein/regenerating protein I (PSP) are highly elevated. The origin of PSP during these conditions is presumably the pancreas, however, an intestinal origin cannot be excluded. Similarly, pancreatitis-associated protein (PAP) was identified in the pancreas. These proteins were also localized in intestinal organs. Here we aim to elucidate the bio-distribution of PSP and PAP in animal models of sepsis and in healthy humans.
PSP and PAP responded to remote lesions in rats although the pancreatic response was much more pronounced than the intestinal. Tissue distribution of PSP demonstrated a 100-fold higher content in the pancreas compared to any other organ while PAP was most abundant in the small intestine. Both proteins responded to CLP or sham operation in the pancreas. PSP also increased in the intestine during CLP. The distribution of PSP and PAP in human tissue mirrored the distribution in the murine models.
Distribution of PSP and PAP was visualized by immunohistochemistry. Rats and mice underwent midline laparotomies followed by mobilization of tissue and incision of the pancreatic duct or duodenum. Standard cecum-ligation-puncture (CLP) procedures or sham laparotomies were performed. Human tissue extracts were analyzed for PSP and PAP.
The pancreas reacts to remote lesions and septic insults in mice and rats with increased PSP synthesis, while PAP is selectively responsive to septic events. Furthermore, our results suggest that serum PSP in septic patients is predominantly derived through an acute phase response of the pancreas.

Keywords
Animals, Biomarkers, Humans, Lithostathine/secretion, Male, Mice, Pancreas/metabolism, Pancreatitis-Associated Proteins/secretion, Protein Transport, Rats, Sepsis/blood, Sepsis/etiology, Sepsis/metabolism, Stress, Physiological, acute phase reaction, intestine, pancreas, regenerating proteins, sepsis
Pubmed
Web of science
Open Access
Yes
Create date
20/04/2017 7:45
Last modification date
20/08/2019 14:27
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