Comparison of three acellular tests for assessing the oxidation potential of nanomaterials

Details

Ressource 1Download: BIB_6624379946A3.P001.pdf (1093.44 [Ko])
State: Public
Version: Author's accepted manuscript
Serval ID
serval:BIB_6624379946A3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Comparison of three acellular tests for assessing the oxidation potential of nanomaterials
Journal
Aerosol Science and Technology
Author(s)
Sauvain Jean-Jacques, Rossi Michel J., Riediker Michael
ISSN
0278-6826 (Print)
1521-7388 (Electronic)
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
47
Number
2
Pages
218-227
Language
english
Abstract
Great effort is put into developing reliable, predictive, high-throughput, and low-cost screening approaches for the toxicity evaluation of ambient and manufactured nanoparticles (NP). These tests often consider oxidative reactivity, as oxidative stress is a well-documented pathway in particle toxicology. Based on a panel of six carbonaceous and five metal/metal oxide (Me/MeOx) nanoparticles, we: (i) compared the specifications (linearity, detection limits, repeatability) of three acellular reactivity tests using either dithiothreitol (DTT assay), dichlorofluorescein (DCFH assay), or ascorbic acid (AA-assay) as the reducing agent; and (ii) evaluated which physicochemical properties were important for explaining the observed reactivity. The selected AA assay was found to be neither sensitive nor robust enough to be retained. For the other tests, the surface properties of carbonaceous NP were of utmost importance for explaining their reactivity. In particular, the presence of "strongly reducing" surface functions explained most of its DCFH reactivity and a large part of its DTT reactivity. For the selected Me/MeOx, a different picture emerged. Whereas all particles were able to oxidize DCFH, dissolution and complexation processes could additionally influence the measured reactivity, as observed using the DTT assay. This study suggests that a combination of the DTT and DCFH assays provides complementary information relative to the quantification of the oxidative capacity of NP.
Keywords
Nanoparticles , Toxicity Tests , Oxidative Stress
Web of science
Open Access
Yes
Create date
11/01/2013 12:13
Last modification date
20/08/2019 14:22
Usage data