Mitotic functions for SNAP45, a subunit of the small nuclear RNA-activating protein complex SNAPc.

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State: Public
Version: Final published version
Serval ID
serval:BIB_56E189826993
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Mitotic functions for SNAP45, a subunit of the small nuclear RNA-activating protein complex SNAPc.
Journal
Journal of Biological Chemistry
Author(s)
Shanmugam M., Hernandez N.
ISSN
0021-9258
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
283
Number
21
Pages
14845-14846
Language
english
Notes
Publication types: Journal Article
Abstract
The small nuclear RNA-activating protein complex SNAP(c) is required for transcription of small nuclear RNA genes and binds to a proximal sequence element in their promoters. SNAP(c) contains five types of subunits stably associated with each other. Here we show that one of these polypeptides, SNAP45, also known as PTF delta, localizes to centrosomes during parts of mitosis, as well as to the spindle midzone during anaphase and the mid-body during telophase. Consistent with localization to these mitotic structures, both down- and up-regulation of SNAP45 lead to a G(2)/M arrest with cells displaying abnormal mitotic structures. In contrast, down-regulation of SNAP190, another SNAP(c) subunit, leads to an accumulation of cells with a G(0)/G(1) DNA content. These results are consistent with the proposal that SNAP45 plays two roles in the cell, one as a subunit of the transcription factor SNAP(c) and another as a factor required for proper mitotic progression.
Keywords
Antibodies/immunology, CDC2 Protein Kinase/metabolism, Centrosome/metabolism, Cyclin B/metabolism, Down-Regulation, Hela Cells, Humans, Mitosis, Protein Binding, Transcription Factors/genetics, Transcription Factors/immunology
Pubmed
Web of science
Open Access
Yes
Create date
18/06/2009 13:16
Last modification date
20/08/2019 14:11
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