Activity of daptomycin- and vancomycin-loaded poly-epsilon-caprolactone microparticles against mature staphylococcal biofilms.

Details

Ressource 1Download: 26185439_BIB_56C781EA4447.pdf (2120.46 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_56C781EA4447
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Activity of daptomycin- and vancomycin-loaded poly-epsilon-caprolactone microparticles against mature staphylococcal biofilms.
Journal
International Journal of Nanomedicine
Author(s)
Ferreira I.S., Bettencourt A.F., Gonçalves L.M., Kasper S., Bétrisey B., Kikhney J., Moter A., Trampuz A., Almeida A.J.
ISSN
1178-2013 (Electronic)
ISSN-L
1176-9114
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
10
Pages
4351-4366
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
The aim of the present study was to develop novel daptomycin-loaded poly-epsilon-caprolactone (PCL) microparticles with enhanced antibiofilm activity against mature biofilms of clinically relevant bacteria, methicillin-resistant Staphylococcus aureus (MRSA) and polysaccharide intercellular adhesin-positive Staphylococcus epidermidis. Daptomycin was encapsulated into PCL microparticles by a double emulsion-solvent evaporation method. For comparison purposes, formulations containing vancomycin were also prepared. Particle morphology, size distribution, encapsulation efficiency, surface charge, thermal behavior, and in vitro release were assessed. All formulations exhibited a spherical morphology, micrometer size, and negative surface charge. From a very early time stage, the released concentrations of daptomycin and vancomycin were higher than the minimal inhibitory concentration and continued so up to 72 hours. Daptomycin presented a sustained release profile with increasing concentrations of the drug being released up to 72 hours, whereas the release of vancomycin stabilized at 24 hours. The antibacterial activity of the microparticles was assessed by isothermal microcalorimetry against planktonic and sessile MRSA and S. epidermidis. Regarding planktonic bacteria, daptomycin-loaded PCL microparticles presented the highest antibacterial activity against both strains. Isothermal microcalorimetry also revealed that lower concentrations of daptomycin-loaded microparticles were required to completely inhibit the recovery of mature MRSA and S. epidermidis biofilms. Further characterization of the effect of daptomycin-loaded PCL microparticles on mature biofilms was performed by fluorescence in situ hybridization. Fluorescence in situ hybridization showed an important reduction in MRSA biofilm, whereas S. epidermidis biofilms, although inhibited, were not eradicated. In addition, an important attachment of the microparticles to MRSA and S. epidermidis biofilms was observed. Finally, all formulations proved to be biocompatible with both ISO compliant L929 fibroblasts and human MG63 osteoblast-like cells.
Keywords
Anti-Bacterial Agents/chemistry, Anti-Bacterial Agents/pharmacokinetics, Biofilms/drug effects, Caproates/chemistry, Daptomycin/chemistry, Daptomycin/pharmacokinetics, Lactones/chemistry, Methicillin-Resistant Staphylococcus aureus/drug effects, Microbial Sensitivity Tests, Microspheres, Vancomycin/chemistry, Vancomycin/pharmacokinetics
Pubmed
Web of science
Open Access
Yes
Create date
24/07/2015 17:23
Last modification date
20/08/2019 14:10
Usage data