Dendritic cell-specific antigen delivery by coronavirus vaccine vectors induces long-lasting protective antiviral and antitumor immunity.

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Version: author
Serval ID
serval:BIB_4DFE2B779A05
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dendritic cell-specific antigen delivery by coronavirus vaccine vectors induces long-lasting protective antiviral and antitumor immunity.
Journal
MBIO
Author(s)
Cervantes-Barragan L., Züst R., Maier R., Sierro S., Janda J., Levy F., Speiser D., Romero P., Rohrlich P.S., Ludewig B., Thiel V.
ISSN
2150-7511
Publication state
Published
Issued date
2010
Volume
1
Number
4
Pages
e00171-10
Language
english
Abstract
Efficient vaccination against infectious agents and tumors depends on specific antigen targeting to dendritic cells (DCs). We report here that biosafe coronavirus-based vaccine vectors facilitate delivery of multiple antigens and immunostimulatory cytokines to professional antigen-presenting cells in vitro and in vivo. Vaccine vectors based on heavily attenuated murine coronavirus genomes were generated to express epitopes from the lymphocytic choriomeningitis virus glycoprotein, or human Melan-A, in combination with the immunostimulatory cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF). These vectors selectively targeted DCs in vitro and in vivo resulting in vector-mediated antigen expression and efficient maturation of DCs. Single application of only low vector doses elicited strong and long-lasting cytotoxic T-cell responses, providing protective antiviral and antitumor immunity. Furthermore, human DCs transduced with Melan-A-recombinant human coronavirus 229E efficiently activated tumor-specific CD8(+) T cells. Taken together, this novel vaccine platform is well suited to deliver antigens and immunostimulatory cytokines to DCs and to initiate and maintain protective immunity.
Pubmed
Web of science
Open Access
Yes
Create date
02/02/2011 9:38
Last modification date
20/08/2019 14:03
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