Fine processes of Nestin-GFP-positive radial glia-like stem cells in the adult dentate gyrus ensheathe local synapses and vasculature.

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Version: Author's accepted manuscript
Serval ID
serval:BIB_4D51115E83F0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Fine processes of Nestin-GFP-positive radial glia-like stem cells in the adult dentate gyrus ensheathe local synapses and vasculature.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Moss J., Gebara E., Bushong E.A., Sánchez-Pascual I., O'Laoi R., El M'Ghari I., Kocher-Braissant J., Ellisman M.H., Toni N.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Publication state
Published
Issued date
03/05/2016
Peer-reviewed
Oui
Volume
113
Number
18
Pages
E2536-45
Language
english
Notes
Publication types: ARTICLE
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Adult hippocampal neurogenesis relies on the activation of neural stem cells in the dentate gyrus, their division, and differentiation of their progeny into mature granule neurons. The complex morphology of radial glia-like (RGL) stem cells suggests that these cells establish numerous contacts with the cellular components of the neurogenic niche that may play a crucial role in the regulation of RGL stem cell activity. However, the morphology of RGL stem cells remains poorly described. Here, we used light microscopy and electron microscopy to examine Nestin-GFP transgenic mice and provide a detailed ultrastructural reconstruction analysis of Nestin-GFP-positive RGL cells of the dentate gyrus. We show that their primary processes follow a tortuous path from the subgranular zone through the granule cell layer and ensheathe local synapses and vasculature in the inner molecular layer. They share the ensheathing of synapses and vasculature with astrocytic processes and adhere to the adjacent processes of astrocytes. This extensive interaction of processes with their local environment could allow them to be uniquely receptive to signals from local neurons, glia, and vasculature, which may regulate their fate.

Keywords
Animals, Astrocytes/cytology, Cells, Cultured, Cerebral Arteries/cytology, Cerebral Arteries/metabolism, Dentate Gyrus/cytology, Dentate Gyrus/metabolism, Green Fluorescent Proteins, Male, Mice, Mice, Transgenic, Nestin/metabolism, Neural Stem Cells/cytology, Neural Stem Cells/metabolism, Neurogenesis/physiology, Neuroglia/cytology, Neuroglia/metabolism, Neurovascular Coupling/physiology, Stem Cell Niche/physiology, Synapses/metabolism, Synapses/ultrastructure, Tissue Distribution
Pubmed
Open Access
Yes
Create date
03/05/2016 16:38
Last modification date
20/08/2019 14:02
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