Novel microsatellite loci for Sebaea aurea (Gentianaceae) and cross-amplification in related species.

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Serval ID
serval:BIB_41CEA22E43C6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Novel microsatellite loci for Sebaea aurea (Gentianaceae) and cross-amplification in related species.
Journal
Applications in Plant Sciences
Author(s)
Kissling J., Bachmann O., Thali M.R., Segarra-Moragues J.G.
ISSN
2168-0450 (Electronic)
ISSN-L
2168-0450
Publication state
Published
Issued date
2013
Volume
1
Number
12
Pages
1300056
Language
english
Abstract
Premise of the study: Microsatellite loci were developed in Sebaea aurea (Gentianaceae) to investigate the functional role of diplostigmaty (i.e., the presence of additional stigmas along the style).
Methods and Results: One hundred seventy-four and 180 microsatellite loci were isolated through 454 shotgun sequencing of genomic and microsatellite-enriched DNA libraries, respectively. Sixteen polymorphic microsatellite loci were characterized, and 12 of them were selected to genotype individuals from two populations. Microsatellite amplification was conducted in two multiplex groups, each containing six microsatellite loci. Cross-species amplification was tested in seven other species of Sebaea. The 12 novel microsatellite loci amplified only in the two most closely related species to S. aurea (i.e., S. ambigua and S. minutiflora) and were also polymorphic in these two species.
Conclusions: These results demonstrate the usefulness of this set of newly developed microsatellite loci to investigate the mating system and population genetic structure in S. aurea and related species.
Keywords
diplostigmaty, Gentianaceae, microsatellites, multiplex, Sebaea aurea
Pubmed
Web of science
Open Access
Yes
Create date
30/10/2014 10:34
Last modification date
20/08/2019 13:42
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