Cell biological characterization of the malaria vaccine candidate trophozoite exported protein 1.

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Serval ID
serval:BIB_24FCFF24E8AE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cell biological characterization of the malaria vaccine candidate trophozoite exported protein 1.
Journal
PLoS One
Author(s)
Kulangara C., Luedin S., Dietz O., Rusch S., Frank G., Mueller D., Moser M., Kajava A.V., Corradin G., Beck H.P., Felger I.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2012
Volume
7
Number
10
Pages
e46112
Language
english
Abstract
In a genome-wide screen for alpha-helical coiled coil motifs aiming at structurally defined vaccine candidates we identified PFF0165c. This protein is exported in the trophozoite stage and was named accordingly Trophozoite exported protein 1 (Tex1). In an extensive preclinical evaluation of its coiled coil peptides Tex1 was identified as promising novel malaria vaccine candidate providing the rational for a comprehensive cell biological characterization of Tex1. Antibodies generated against an intrinsically unstructured N-terminal region of Tex1 and against a coiled coil domain were used to investigate cytological localization, solubility and expression profile. Co-localization experiments revealed that Tex1 is exported across the parasitophorous vacuole membrane and located to Maurer's clefts. Change in location is accompanied by a change in solubility: from a soluble state within the parasite to a membrane-associated state after export to Maurer's clefts. No classical export motifs such as PEXEL, signal sequence/anchor or transmembrane domain was identified for Tex1.
Pubmed
Web of science
Open Access
Yes
Create date
07/12/2012 10:41
Last modification date
20/08/2019 13:03
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