TRAIP is a regulator of the spindle assembly checkpoint.

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Version: Author's accepted manuscript
Secondary document(s)
Download: 5149.full.pdf (9055.03 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_0B0118FCA445
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
TRAIP is a regulator of the spindle assembly checkpoint.
Journal
Journal of Cell Science
Author(s)
Chapard C., Meraldi P., Gleich T., Bachmann D., Hohl D., Huber M.
ISSN
1477-9137 (Electronic)
ISSN-L
0021-9533
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
127
Number
24
Pages
5149-5156
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
Accurate chromosome segregation during mitosis is temporally and spatially coordinated by fidelity-monitoring checkpoint systems. Deficiencies in these checkpoint systems can lead to chromosome segregation errors and aneuploidy, and promote tumorigenesis. Here, we report that the TRAF-interacting protein (TRAIP), a ubiquitously expressed nucleolar E3 ubiquitin ligase important for cellular proliferation, is localized close to mitotic chromosomes. Its knockdown in HeLa cells by RNA interference (RNAi) decreased the time of early mitosis progression from nuclear envelope breakdown (NEB) to anaphase onset and increased the percentages of chromosome alignment defects in metaphase and lagging chromosomes in anaphase compared with those of control cells. The decrease in progression time was corrected by the expression of wild-type but not a ubiquitin-ligase-deficient form of TRAIP. TRAIP-depleted cells bypassed taxol-induced mitotic arrest and displayed significantly reduced kinetochore levels of MAD2 (also known as MAD2L1) but not of other spindle checkpoint proteins in the presence of nocodazole. These results imply that TRAIP regulates the spindle assembly checkpoint, MAD2 abundance at kinetochores and the accurate cellular distribution of chromosomes. The TRAIP ubiquitin ligase activity is functionally required for the spindle assembly checkpoint control.
Pubmed
Web of science
Open Access
Yes
Create date
17/01/2015 11:05
Last modification date
20/08/2019 12:32
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