Microsatellites can be misleading: an empirical and simulation study.

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Serval ID
serval:BIB_08574EED056C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Microsatellites can be misleading: an empirical and simulation study.
Journal
Evolution
Author(s)
Balloux F., Brünner H., Lugon-Moulin N., Hausser J., Goudet J.
ISSN
0014-3820[print], 0014-3820[linking]
Publication state
Published
Issued date
2000
Volume
54
Number
4
Pages
1414-1422
Language
english
Abstract
It has been long recognized that highly polymorphic genetic markers can lead to underestimation of divergence between populations when migration is low. Microsatellite loci, which are characterized by extremely high mutation rates, are particularly likely to be affected. Here, we report genetic differentiation estimates in a contact zone between two chromosome races of the common shrew (Sorex araneus), based on 10 autosomal microsatellites, a newly developed Y-chromosome microsatellite, and mitochondrial DNA. These results are compared to previous data on proteins and karyotypes. Estimates of genetic differentiation based on F- and R-statistics are much lower for autosomal microsatellites than for all other genetic markers. We show by simulations that this discrepancy stems mainly from the high mutation rate of microsatellite markers for F-statistics and from deviations from a single-step mutation model for R-statistics. The sex-linked genetic markers show that all gene exchange between races is mediated by females. The absence of male-mediated gene flow most likely results from male hybrid sterility.
Keywords
Animals, Computer Simulation, DNA, Mitochondrial/genetics, Female, Genetic Markers, Genotype, Karyotyping, Male, Microsatellite Repeats, Models, Genetic, Models, Statistical, Mutation, Proteins/genetics, Shrews/classification, Shrews/genetics, Y Chromosome
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 17:10
Last modification date
20/08/2019 12:30
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