Book/Dissertation / PhD Thesis

FZJ-2022-03687

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Hybrid hydrogels promote physiological functionality of long-term cultured primary neuronal cells in vitro

Meessen, C. (Corresponding author)FZJ*

2022
Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag Jülich
ISBN: 978-3-95806-643-4

Please use a persistent id in citations: http://hdl.handle.net/2128/32735  urn:nbn:de:0001-2022112346

Abstract: This study aimed to develop and establish a modular system for the 3D cultivation of primary neuronal cells, with the desirable tuneability in mechanical and chemical properties, while enhancing the cell viability for long-term studies. It could be shown that the novel Collagen-PVP-co-GMA3mol% biohybrid hydrogel promote physiological development of functional cortical networks. Primary cortical cells had been transfected with GCaMP6f to record spontaneous neuronal activity over 40 days in vitro. Network dynamics had been estimated based on generalizedtransfer entropy (GTE), showing the development of a functional network of balanced segregation and integration, with a strong emerging ability for resilience over 40 days in culture. Synchronous network activity could be observed already at day in vitro (DIV) 8-9 in absenceof external stimuli. Further, a correlation of network maturation as well as functional development with increasing molecular weight of PVP could be shown. With decreasing polymer chain length, neurite outgrowth and network maturation are significantly promoted. The addition ofPVP to collagen was demonstrated to not only support network functionality and growth, but also prevent the degradation of the hydrogel

Note: Dissertation, RWTH Aachen, 2021

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Contributing Institute(s):

1. Bioelektronik (IBI-3)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2022

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Database coverage:
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