Book/Dissertation / PhD Thesis

FZJ-2022-03455

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Establishing regulatable expression systems in the acetic acid bacterium Gluconobacter oxydans 621H

Fricke, P. M. (Corresponding author)FZJ*

2022
Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag Jülich
ISBN: 978-3-95806-642-7

Please use a persistent id in citations: http://hdl.handle.net/2128/32733  urn:nbn:de:0001-2022112326

Abstract: Among industrially relevant microorganisms, the acetic acid bacterium Gluconobacter oxydans is valued for its ability to incompletely oxidize a vast number of carbohydrates stereoand regio-specifically. Biotechnological production processes involving G. oxydans strains so far utilized exclusively constitutive target gene expression. Regulatable promoters used in G. oxydans suffered from low induction fold-changes and relatively high basal promoter activity already when not induced. This study aimed to establish regulatable promoter systems in G. oxydans that allow tuned target gene expression in an effector-dependent manner. For this purpose, expression plasmids were constructed to test four well-characterized heterologous regulator-promoter pairs in G. oxydans. Additionally, screenings were performed to identify regulatable endogenous G. oxydans promoters responding to a metabolite given as an external stimulus and suitable for controlled gene expression.

Note: Dissertation, Univ. Düsseldorf, 2022

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Contributing Institute(s):

1. Biotechnologie (IBG-1)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2022

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Database coverage:
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