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130. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

30.04. - 03.05.2013, München

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Mesenchymal stromal cells improve the wound healing potential of surgical sutures

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  • Ann Katharin Reckhenrich - Klinikum rechts der Isar - TUM, Klinik für Plastsiche Chirurgie und Handchirurgie, München
  • Manuela Kirsch - Klinikum rechts der Isar - TUM, Klinik für Plastsiche Chirurgie und Handchirurgie, München
  • Elizabeth Wahl - Klinikum rechts der Isar - TUM, Klinik für Plastsiche Chirurgie und Handchirurgie, München
  • Thilo Ludwig Schenck - Klinikum rechts der Isar - TUM, Klinik für Plastsiche Chirurgie und Handchirurgie, München
  • Farid Rezaeian - Klinikum rechts der Isar - TUM, Klinik für Plastsiche Chirurgie und Handchirurgie, München
  • Yves Harder - Klinikum rechts der Isar - TUM, Klinik für Plastsiche Chirurgie und Handchirurgie, München
  • Hans Günther Machens - Klinikum rechts der Isar - TUM, Klinik für Plastsiche Chirurgie und Handchirurgie, München
  • José Tomàs Egaña - Klinikum rechts der Isar - TUM, Klinik für Plastsiche Chirurgie und Handchirurgie, München

Deutsche Gesellschaft für Chirurgie. 130. Kongress der Deutschen Gesellschaft für Chirurgie. München, 30.04.-03.05.2013. Düsseldorf: German Medical Science GMS Publishing House; 2013. Doc13dgch200

doi: 10.3205/13dgch200, urn:nbn:de:0183-13dgch2002

Published: April 26, 2013

© 2013 Reckhenrich et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Introduction: Delayed wound healing and scar formation are among the most frequent complications after surgical interventions. Besides esthetic impairments, patients suffer loss of tissue functionality. Although biodegradable surgical sutures present an excellent opportunity to locally deliver bioactive molecules, their primary function is mechanical tissue fixation. Here we present a strategy to bioactivate surgical sutures by seeding of mesenchymal stromal cells (MSC), which play a crucial role in wound healing acting as trophic mediators.

Material and methods: MSCs were isolated from human fat tissue and further characterized. Afterwards sutures were seeded with cells and their distribution and viability was analyzed. Further the potential to secrete paracrine factors was tested and their bioactivity was confirmed in vitro.

Results: Histological analysis showed that MSCs attached to the suture material and distributed homogeneously throughout the woven suture material. MTT assay showed that seeded cells survive in the suture and showed a significant increase of metabolic activity after 7 days, suggesting cell proliferation. The analysis of conditioned media showed that seeded sutures released several cytokines that are related to tissue remodeling, immunomodulation and angiogenesis. After characterizing the bioactive sutures in vitro, we were able to show that cells remain in the suture and stay metabolically active after suturing in an ex vivo human wound model.

Conclusion: MSC-seeded sutures represent a promising approach to promote wound healing and decrease scar formation. Further experiments are under investigation to evaluate their effects in vivo.